FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001745346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001745346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106047
Sequences flagged as poor quality0
Sequence length126
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN172451.5591561660580426No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT33960.3070393934434974No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA31820.2876912102288601No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC23780.21499990506732536No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC22880.2068628186686461No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC20330.18380774053905485No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG19550.17675559899353283No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA18570.16789521602608207No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC17870.16156637104933155No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT17620.15930606927192062No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC17520.15840194856095627No Hit
CCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCA17140.1549662898592917No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC17050.15415258121942377No Hit
CTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAA16550.14963197766460196No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC15650.14149489126592268No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC15290.13824005670645098No Hit
CTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATC14490.1310070910187361No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA14480.13091667894763967No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC14390.13010297030777174No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT14220.12856596509913232No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA14170.12811390474365011No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT14090.12739060817487863No Hit
ATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGC13910.1257631908951428No Hit
CTTTGCCGGAAAGCAGCTGGAAGATGGGCGCACCCTGTCTGACTACAACA13550.1225083563356711No Hit
AGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGC13390.12106176319812811No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT13230.11961517006058513No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT13180.11916310970510294No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA13180.11916310970510294No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC13010.11762610449646353No Hit
GGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGA12790.11563703893234192No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG12710.11491374236357044No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT12630.11419044579479896No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT12600.11391920958150965No Hit
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTC12600.11391920958150965No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC12440.11247261644396667No Hit
TGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTG12370.11183973194629161No Hit
CACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGAC12200.11030272673765219No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAC12150.10985066638217003No Hit
CTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACA12090.10930819395559141No Hit
CTTTGCCGGAAAACAGCTGGAAGATGGTCGTACCCTGTCTGACTACAACA12010.10858489738681991No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT11980.1083136611735306No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATT11870.1073191283914698No Hit
CATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAA11740.10614377146721612No Hit
CTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTG11540.10433553004528741No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC11330.10243687655226225No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC11230.10153275584129787No Hit
CTACAACATCCAGAAAGAGTCCACCTTGCACCTGGTGCTCCGTCTCAGAG11220.10144234377020143No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC11170.10099028341471927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)