FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001745353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001745353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726108
Sequences flagged as poor quality0
Sequence length126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG123850.3323843538619922No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA114280.3067007182829913No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA110290.2959924940447244No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT104420.28023879071674784No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC72580.1947876980484731No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG68470.18375742195341627No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC68120.18281810403777882No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT68070.18268391576411633No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA67010.17983912436247151No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA66870.17946339719621654No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA66540.17857775459004407No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG66090.17737006012708165No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT58500.15700028018511542No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC57930.155470533865363No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG54220.1455137639596061No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC50920.13665733789788165No Hit
GTCTTCACGAAGATCTGCATCCCACCTCTAAGACGGAGCACCAGGTGCAG50500.13553015639911672No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG48340.12973322297689707No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA47150.12653954206372978No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC45680.1225944068180525No Hit
CTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTGCTCAGTTACCTGG42970.11532140238554546No Hit
CCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAG42730.1146772986719655No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT42660.114489435088838No Hit
CTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGG42360.11368430544686306No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT41880.11239609801970314No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC41340.11094686466414823No Hit
CTTCCATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGT41310.11086635169995072No Hit
CCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGG40620.10901455352340834No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC39600.10627711274069351No Hit
CTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGA39470.10592822322917103No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG39210.10523044420612607No Hit
CCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT38900.10439847690941863No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG38840.10423745098102363No Hit
GGACTCTTTCTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCT37680.10112428303205381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)