FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001745357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001745357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4761498
Sequences flagged as poor quality0
Sequence length126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA150340.31574097059370815No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG115990.2435998082956246No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA101730.2136512500897827No Hit
GTCCACTTTGTTTCCAGAACCTTGTTCTGCTGCTCCAGGAACCGCACCTT93000.1953166839511431No Hit
GCTCGAACAACGGCTCCAGGTTCTGCCTCACAGTCTTGGTGCCCTGCTCC92850.19500165704154448No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG92620.19451861578015994No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG86510.18168651966250957No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT84870.17824222545089802No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT83540.17544898685245694No Hit
GTCTGCCTTGGCTTGCAGTTCAACCTTGTTCATGTAGGCAGCATCCACAT79310.16656522800177592No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC78600.16507410062967579No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG76610.1608947436290008No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG74560.15658937586448635No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT70100.1472225757524208No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT68220.14327423848545143No Hit
GCCTTGACCTCAGCGATGATGCTGTCCAGGTCCAGGTTGCGGTTGTTGTC64890.13628064109236213No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC59760.12550672078408937No Hit
TACTGCTCGAACAACGGCTCCAGGTTCTGCCTCACAGTCTTGGTGCCCTG58710.12330153241689905No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC58390.12262947500975532No Hit
GTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCAACACGCTGTTGCT56740.11916417900417055No Hit
GTGCCCTGCTCCTGCAGCAGGGTCCACTTTGTTTCCAGAACCTTGTTCTG56400.11845011800908033No Hit
GCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACAT51710.10860027663563021No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG51460.10807523178629919No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT50620.10631108109254693No Hit
GCCTCACAGTCTTGGTGCCCTGCTCCTGCAGCAGGGTCCACTTTGTTTCC49010.1029297922628551No Hit
CTCCAGGTTCTGCCTCACAGTCTTGGTGCCCTGCTCCTGCAGCAGGGTCC48930.10276177791106915No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)