FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001745362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001745362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4311114
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1987914.611128353367598No Hit
GGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGTT211110.4896878161885768No Hit
AGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGTTGCT180220.4180358023471427No Hit
CGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGC169300.3927059224135571No Hit
GAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCG111060.2576132294344339No Hit
GAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGTTGCTT107930.2503529250212358No Hit
GGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGTTGCTTCGCT107830.2501209664137854No Hit
GTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGTTGCTTCGCTG103220.2394276746103211No Hit
AAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGTTGC103050.23903334497765544No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC73940.17151019434883885No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC73840.17127823574138842No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA63960.14836072532528716No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT63740.14785041638889623No Hit
GTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGTTGCTTCGCTGCAACT57870.13423444613155672No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA54590.126626203807183No Hit
GAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGTTGCTTCG52850.12259012403754575No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC49750.11539940720658279No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT49740.11537621134583775No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG48950.11354373834697945No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC48050.1114561108799257No Hit
GCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTG47950.11122415227247527No Hit
AGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGT46780.1085102365653054No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT46380.10758240213550373No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT43300.10043807702603087No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)