FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001745373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001745373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345703
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA56710.42141542375992325No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG55300.4109376288824503No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG48460.3601091771364112No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG35280.2621678037427278No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG32730.24321860024091496No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT31110.23118028272211627No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT25200.18726271695909127No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA24660.18324994445282503No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT23970.1781225129170404No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC22480.16705023322382426No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT22460.16690161201988848No Hit
GGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATC22450.1668273014179206No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG20760.15426880968534662No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG19410.14423687841968102No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC18730.13918375748586426No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC18680.1388122044760248No Hit
GCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGG18610.13829203026224954No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC18300.13598840160124484No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT17530.13026648524971707No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC17170.1275913035788729No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG16590.12328128866473509No Hit
GTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCAACACGCTGTTGCT16470.12238956144112036No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT16250.12075472819782672No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC16150.12001162217814777No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT15550.11555298606007416No Hit
GCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACAT15300.11369522101087684No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC15260.11339797860300527No Hit
GGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAATAACA14970.11124297114593637No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC14940.11102003934003268No Hit
ATCCATGTCAACAGCCAAGCCAACACGCTGTTGCTCCAATACATAAAAGG14840.11027693332035374No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC14230.10574398660031226No Hit
GGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAG13460.10002207024878446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)