FastQCFastQC Report
Fri 31 May 2024
EGAF00001745374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001745374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345703
Total Bases169.5 Mbp
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA33770.25094690284557586No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT26170.19447084534997694No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG25060.18622236853154076No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC23080.17150886934189788No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT22670.16846213466121424No Hit
GCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAA22030.16370625613526907No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC20910.155383468714865No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT20180.1499587947712088No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA19700.14639188587674992No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT19590.1455744692551031No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC17750.13190131849301073No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG17400.12930044742413443No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA17120.12721975056903342No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG14860.11042555452428952No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC14380.10685864562983065No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT14280.10611553961015173No Hit
AGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAG14000.1040348427550507No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT13980.10388622155111493No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC13880.10314311553143599No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC13710.1018798352979818No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTG13680.10165690349207812No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC13610.10113672927830288No Hit
GTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAAT13470.10009638085075237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)