FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001745383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001745383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5628270
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG190010.3375992978304168No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG130660.2321494882086325No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA130560.23197181371895806No Hit
GCCAGCATTTGGGTAGTATTCTGGGGTCCGACTATGCGAGTGTGGTGTCA128290.2279386028033481No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT111080.19736082313037578No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA106550.18931216874812332No Hit
CTCGTATAACAATAAGGAGCTGCCACAGCATCTGGATTCCTGCAGTAGTT104920.18641607456642983No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT101750.18078379324374985No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT97090.17250416202492064No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT96570.17158025467861351No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG94740.16832881151757112No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT92930.16511290325446362No Hit
CCAGCATTTGGGTAGTATTCTGGGGTCCGACTATGCGAGTGTGGTGTCAT89650.15928517999314176No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG87930.15622917877074127No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT86920.15443466642502937No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC82690.14691903551180024No Hit
GTCCGACTATGCGAGTGTGGTGTCATAGATGACCAAGCTTGGCAGGTTCT80430.14290359204515776No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG76630.13615196143752878No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC72780.12931149358506255No Hit
CTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGA71720.1274281439945134No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAA70740.12568693399570383No Hit
GTGGAGTATGTGCCTCGATAACTCTGTCCATTACCATGGTAGCACTCCTG66540.11822460542937704No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC65740.11680320951198149No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTG65470.11632348838986047No Hit
GTCATAGATGACCAAGCTTGGCAGGTTCTTCCTGTGACAGTGGTGGAGTA63700.11317864992262275No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA62250.11060236982234328No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG62240.11058460237337583No Hit
GTCGGAGGCGCGACGGCAGTCCCTTCTGCGTCTGAGCATTGCGTCAGGTT61700.10962516012913383No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC60360.10724432196749623No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT60180.10692450788608224No Hit
GTCCATTACCATGGTAGCACTCCTGCACCCCAGGCCTTTGCTCAGTCGGT58270.10353092513330028No Hit
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAA58110.10324664594982118No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC56880.10106124972682547No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)