FastQCFastQC Report
Thu 2 May 2024
EGAF00001748689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001748689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38821106
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT659359416.98455989378561Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14338253.69341615357378No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1814360.46736432496281793TruSeq Adapter, Index 8 (100% over 50bp)
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1300740.3350600057607838Clontech SMART CDS Primer II A (96% over 26bp)
ATGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT920090.23700767309411536Clontech SMART CDS Primer II A (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT788260.20304934125266808Clontech SMART CDS Primer II A (100% over 25bp)
AAGCTGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT570780.1470282685918325Clontech SMART CDS Primer II A (96% over 26bp)
AAGCGGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT454400.11704973062848854Clontech SMART CDS Primer II A (96% over 26bp)
AAGTAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT408950.10534218164727199Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)