FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001748697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001748697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25372558
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT343372813.53323539550092Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2325360.9164862289407321No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC854950.33695853606877163TruSeq Adapter, Index 12 (100% over 50bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT439610.17326199431685207Clontech SMART CDS Primer II A (100% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296140.11671665111574481No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)