FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001753971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001753971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9878264
Sequences flagged as poor quality0
Sequence length50
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG5518985.586993828065336TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC3180513.21970540572716TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATC2910872.9467424640604865TruSeq Adapter, Index 8 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATG320340.32428774934543153TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATC276960.2803731505859734TruSeq Adapter, Index 8 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC196520.19894183836350193TruSeq Adapter, Index 8 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGAATC161950.16394581072139802TruSeq Adapter, Index 8 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGCATC114930.11634635397474698TruSeq Adapter, Index 8 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGCATG107310.10863244796859044TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATG105030.1063243501084806TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGCATCTCGTATG102520.10378341781511408TruSeq Adapter, Index 8 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)