FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001753991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001753991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15040975
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC7172924.768919568046619TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG3886642.584034612117898TruSeq Adapter, Index 5 (100% over 49bp)
TCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAA290710.19327869370170486TruSeq Adapter, Index 5 (100% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC162230.10785869931969172TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)