FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001753992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001753992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15040975
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3715382.4701723126326582Illumina Single End PCR Primer 1 (100% over 50bp)
TCTGAACTCCAGTCAAGCAGAAGACGGCATACGAGATCACTGTGTGACTG266880.1774353058894121Illumina PCR Primer Index 5 (100% over 37bp)
ACTCCAGTCAAGCAGAAGACGGCATACGAGATCACTGTGTGACTGGAGTA159470.10602371189367711Illumina PCR Primer Index 5 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)