FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001754200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17070037
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1243530.728486997421271Illumina Single End PCR Primer 1 (100% over 50bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC661420.3874742626509831No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG249220.14599851189543409Illumina Single End PCR Primer 1 (100% over 50bp)
CCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGAC191970.11246021317938562No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA174280.10209702533157954No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC173940.10189784591562398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)