FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001754206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15913321
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC973450.6117202059834022No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC639160.401650918749141Illumina Single End PCR Primer 1 (100% over 50bp)
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA308110.19361766158050855No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC248560.1561961830594632No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC246990.15520958824371103No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA224750.1412338756944575No Hit
CCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGAC213110.13391924916238412No Hit
CTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGG203280.12774203448796137No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC169170.1063071624081485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)