FastQCFastQC Report
Tue 27 Sep 2022
EGAF00001754213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20364380
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT3992921.9607373266458394TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA2545221.2498391799799453TruSeq Adapter, Index 15 (97% over 40bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC449640.2207972940988137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)