FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001754244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22251860
Sequences flagged as poor quality0
Sequence length50
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGNN297780.1338225209038705Illumina Single End PCR Primer 1 (97% over 48bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGANN243510.10943354847639704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)