FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001754271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19562364
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC214497710.964814886380807TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG15136087.737347081365013TruSeq Adapter, Index 3 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATATCGTATGC256590.1311651291224312TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGCTGAACTCCAGTCACTTAGGCATCTCGTATGCC207650.10614770280319905TruSeq Adapter, Index 3 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATATCGTATG201150.10282499599741626TruSeq Adapter, Index 3 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)