FastQCFastQC Report
Fri 31 Mar 2023
EGAF00001754273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21500459
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG13251206.16321725968734TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC10508574.88760263211125TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG532850.24783191837904484TruSeq Adapter, Index 4 (97% over 49bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG366370.17040101329929747Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC366170.17030799202937946TruSeq Adapter, Index 4 (98% over 50bp)
CACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCT263700.12264854438688962TruSeq Adapter, Index 4 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)