FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001754299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27142177
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT5312481.9572785189633093TruSeq Adapter, Index 27 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA4671791.7212289198467758TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)