FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001754379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20169783
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC12260886.078835850638551TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG6049812.9994422845302795TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC265400.13158297240976763TruSeq Adapter, Index 5 (98% over 50bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC264840.13130532936323608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)