FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001754387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16397911
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT6076743.705801306032214TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA4334692.643440374813597TruSeq Adapter, Index 13 (97% over 40bp)
CACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTG253170.15439161732247478TruSeq Adapter, Index 13 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT240480.14665282669237562TruSeq Adapter, Index 13 (97% over 40bp)
AGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTC237190.14464647356605365TruSeq Adapter, Index 13 (96% over 31bp)
AGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCT219110.13362067887793755TruSeq Adapter, Index 13 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTC217510.13264494483474146TruSeq Adapter, Index 13 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTA177950.10851992061671759TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)