FastQCFastQC Report
Fri 31 Mar 2023
EGAF00001754401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20596671
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3537801.7176562173566785TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2880321.3984395827850045TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)