FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001754441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19119352
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTA11261815.890267619948626TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT4814312.518029899758109TruSeq Adapter, Index 21 (97% over 40bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC302590.15826373195074814No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATATCGTA290550.1519664473984265TruSeq Adapter, Index 21 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)