FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001754529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15015009
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2292811.5270120717210358TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA1998551.3310348332125541TruSeq Adapter, Index 13 (97% over 40bp)
CACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTG428540.2854077543343464TruSeq Adapter, Index 13 (96% over 29bp)
AGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCT328870.2190275077424196TruSeq Adapter, Index 13 (96% over 33bp)
AGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTC312840.2083515234656203TruSeq Adapter, Index 13 (96% over 31bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTC290040.19316671738258698TruSeq Adapter, Index 13 (97% over 34bp)
ACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGC267160.17792863127820968TruSeq Adapter, Index 13 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)