FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001754537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17000253
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG5654453.326097558665744TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC3503792.061022268315654TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATG183970.1082160365495737TruSeq Adapter, Index 8 (97% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)