FastQCFastQC Report
Fri 10 May 2024
EGAF00001754541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18526322
Total Bases926.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3791472.0465314162195822TruSeq Adapter, Index 1 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1859081.0034803454242025TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG314990.17002295436730508TruSeq Adapter, Index 1 (97% over 49bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT221490.11955422128580082No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC190890.10303718136821761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)