FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001754581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19118375
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA4788162.504480637083434TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1690970.8844737065780958TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTA362580.18965000948040825TruSeq Adapter, Index 19 (97% over 40bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC322250.16855512040118473No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG213190.11151052325315307Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)