FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001754595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21072800
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC258343612.259576325879808TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG6850613.250925363501765TruSeq Adapter, Index 9 (100% over 49bp)
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT936710.4445114080710679TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC648640.30780911886412815TruSeq Adapter, Index 9 (98% over 50bp)
AGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTG603470.2863739038001595TruSeq Adapter, Index 9 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT578520.2745339964314187TruSeq Adapter, Index 9 (100% over 50bp)
ACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTT471560.2237766219961277TruSeq Adapter, Index 9 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC445160.211248623818382TruSeq Adapter, Index 9 (100% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT417490.198117953001025TruSeq Adapter, Index 9 (100% over 50bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG394150.18704206370297255Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC305740.14508750616909002No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC305280.1448692152917505TruSeq Adapter, Index 9 (97% over 46bp)
GATCGCAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC232740.11044569302608102TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)