FastQCFastQC Report
Mon 6 May 2024
EGAF00001754813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25081675
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG2334230.9306515613490727TruSeq Adapter, Index 10 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1824610.7274673641214153TruSeq Adapter, Index 10 (100% over 50bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC399330.15921185487013925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)