FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001754951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001754951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19962074
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1226580.6144551913794127TruSeq Adapter, Index 25 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA783140.39231394493377797TruSeq Adapter, Index 25 (97% over 44bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC220340.11037931228989532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)