FastQCFastQC Report
Fri 31 Mar 2023
EGAF00001755013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001755013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20942251
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT2203841.0523415080833478TruSeq Adapter, Index 25 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA1373050.6556363019429TruSeq Adapter, Index 25 (97% over 44bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC221370.10570496934641839No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)