FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001755117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001755117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16921732
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT8265554.8845768270056515TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTA4685332.76882413691459TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGAT470730.2781807441460484TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGA275900.16304477579481816TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGCAT216270.127806066187551TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTT197890.11694429388197378TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTTT172230.10178036149018316TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)