FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001755161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001755161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18466175
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG6518633.530038028990844TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC5362732.904082735054769TruSeq Adapter, Index 7 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTC193020.10452624866817302TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)