FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001755997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001755997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20928218
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA1813640.8666003001306657TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT484420.2314673901045947TruSeq Adapter, Index 27 (97% over 44bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC244350.11675623791762872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)