FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001756029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24763723
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG9988164.033383833279027TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC5700662.3020205806695544TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATG474260.1915140142699868TruSeq Adapter, Index 8 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)