FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001756033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14703146
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG7010464.7679999912943805TruSeq Adapter, Index 1 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC5276743.5888509846804215TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG450520.3064106144358493TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC322980.2196672739289945TruSeq Adapter, Index 1 (98% over 50bp)
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC186240.12666676913906724TruSeq Adapter, Index 1 (100% over 42bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)