FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001756157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18864677
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC6859683.6362562687927285TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG5030172.666449046543442TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC191290.1014011530650644TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)