FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001756163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16318457
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG3544152.171865881682318TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC2057751.260995448282886TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC221770.13590132939652322TruSeq Adapter, Index 9 (97% over 46bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)