FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001756165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13965319
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC6975704.9950165835810845TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1542341.104407282067814TruSeq Adapter, Index 9 (100% over 49bp)
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT296100.21202523193347747TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC209440.14997151157091362TruSeq Adapter, Index 9 (98% over 50bp)
ACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTT204970.14677072539481553TruSeq Adapter, Index 9 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT150310.10763091054346843TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC147370.1055256954746254TruSeq Adapter, Index 9 (97% over 46bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)