FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001756185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18638996
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT3454131.8531738512095823TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA2933711.5739635332289357TruSeq Adapter, Index 13 (97% over 40bp)
CACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTG281530.1510435433324842TruSeq Adapter, Index 13 (96% over 29bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTC242950.13034500356135062TruSeq Adapter, Index 13 (97% over 34bp)
AGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTC204080.10949087601070358TruSeq Adapter, Index 13 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)