FastQCFastQC Report
Fri 3 May 2024
EGAF00001756279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25256612
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA3630711.4375285172848995TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT3041281.2041520058193078TruSeq Adapter, Index 13 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCT286500.11343564212017035TruSeq Adapter, Index 13 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTC261060.10336303222300758TruSeq Adapter, Index 13 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTA259050.10256720101651005TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)