FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001756505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15641946
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG12499467.990987822103465TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC9795846.26254559375157TruSeq Adapter, Index 4 (100% over 50bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG2481481.586426650494766Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG803760.5138491080329776TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC540180.34534066285614334TruSeq Adapter, Index 4 (98% over 50bp)
CACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCT508010.32477416812460547TruSeq Adapter, Index 4 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT333780.21338777157266753TruSeq Adapter, Index 4 (100% over 50bp)
AGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTG320990.20521103959826995TruSeq Adapter, Index 4 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTC275680.17624405556699915TruSeq Adapter, Index 4 (100% over 50bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG265350.16964001793638722TruSeq Adapter, Index 4 (97% over 49bp)
GATCGAAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC220400.14090318429688992TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC204060.1304569137369481TruSeq Adapter, Index 4 (97% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)