FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001756506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15641946
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC11101927.097531215105844Illumina Single End PCR Primer 1 (100% over 50bp)
CAAGCAGAAGACGGCATACGAGATTGGAAGAGCGTCGTGTAGGGAAAGAG835760.5343069206350667Illumina Single End PCR Primer 1 (96% over 30bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCC818580.52332363249432Illumina Single End PCR Primer 1 (98% over 50bp)
CAAGCAGAAGACGGCATACGAGATTGGTCAGTGACTGGAGTTCAGACGTG186590.11928822666949497TruSeq Adapter, Index 4 (100% over 50bp)
CAAGCAGAAGACGGCATACGAGATTGGAAGAGCGTCGGGTAGGGAAAGAG172270.11013335553006001RNA PCR Primer, Index 27 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)