FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001756633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20695767
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC5603922.7077614470630635TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG3543011.7119491150050155TruSeq Adapter, Index 5 (100% over 49bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC775910.3749124156645173No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA369910.17873703351994638No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC319050.15416195978626934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)