FastQCFastQC Report
Tue 27 Sep 2022
EGAF00001756639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21094383
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG4489532.128305909682213TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC2179861.0333841004024626TruSeq Adapter, Index 8 (100% over 50bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC404490.19175246794371753No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA223260.10583860167893984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)