FastQCFastQC Report
Mon 6 May 2024
EGAF00001756640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21094383
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4683662.2203351479870257Illumina Single End PCR Primer 1 (100% over 50bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC358050.16973712859958975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)