FastQCFastQC Report
Wed 1 May 2024
EGAF00001756747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28691069
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG5972052.0815013898575896TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3441701.1995718946547445TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG368780.12853477156950827TruSeq Adapter, Index 4 (97% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)