FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001756807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17306742
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG7623144.404722737531998TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC7026194.059799354494335TruSeq Adapter, Index 4 (100% over 50bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG265640.15348931647562553Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC194110.11215860269945667No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG191730.1107834160814323TruSeq Adapter, Index 4 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)