FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001756817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13365461
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC11328958.476288247745439TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG2895222.1661953897437582TruSeq Adapter, Index 9 (100% over 49bp)
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT269860.20190848635898156TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC229140.17144189788889438TruSeq Adapter, Index 9 (98% over 50bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC213020.1593809596242135No Hit
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT196640.14712549009719902TruSeq Adapter, Index 9 (100% over 50bp)
AGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTG191970.14363140934682314TruSeq Adapter, Index 9 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC156450.11705544612340718TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC156360.11698810837875327TruSeq Adapter, Index 9 (97% over 46bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)