FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001756857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17624684
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA10636776.035155013275699TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT5083942.884556681980795TruSeq Adapter, Index 16 (97% over 40bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC579930.3290441973314245No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTA507580.2879938159458632TruSeq Adapter, Index 16 (97% over 40bp)
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA251030.14243092244944647No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTAT232200.13174704295407508TruSeq Adapter, Index 16 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)